Biology reagent for cells, Extraction reagents for cells

Top Life Science Biology reagent for cells, Extraction reagents for cells

Biology reagent for cells, Extraction reagents for cells

RIPA buffer [R0246] is used to extract proteins from cultured mammalian cells.
The protein can be extracted by simply dissolving cultured mammalian cells in this product. The extracted proteins can be used for applications such as Western blotting. The extracted proteins can be used directly for applications such as Western blotting.
This product contains no protease inhibitors. Protease inhibitors are not included in this product.

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Product code Product name Package SDS / Protocol
R0246RIPA Buffer (Ready-to-use) [for Protein extraction]100 mlSDSProtocol

Product information

Biology reagent for cells, Extraction reagents for cells

Extraction Buffer for Mammalian Cells


This product is supplied as a ready-to-use solution for the lysis of the cultured mammalian cells. Proteins can be extracted by adding this buffer [R0246] to the cells and the extract can be used directly for further analysis such as western blotting. This product does not include protease inhibitors. Please add a protease inhibitor cocktail, if necessary.




Product


R0246 RIPA Buffer (Ready-to-use) [for Protein extraction]




Application




Add the following protease inhibitors to RIPA buffer [R0246].

Leupeptin 10 µg/mL
Pepstatin A 1 µg/mL
Aprotinin 3 µg/mL
AEBSF 1 mM
1. Wash the cultured mouse myeloma-derived cell sp2/0 twice with PBS.
2. Remove PBS and add 200 µL of either cold RIPA buffer [R0246] containing protease inhibitors or the other manufacturer's RIPA buffer containing the same protease inhibitors to 1.0 x 106 cells.
3. Incubate the cells for 15 minutes on ice.
4. Centrifuge the cells at 10,000 x g for 10 minutes at 4 °C
5. Measure the protein concentration of the supernatants.
6. Analyze the supernatants using western blotting.





The extracts were transferred to a PVDF membrane after electrophoresis.
Anti-β actin antibody was used for detection.
Equal or better detection was observed than that of the other manufacturer's product.


References

Not available