Lactate Assay Kit-WST
Lactate Assay Kit-WST
Lactate Assay Kit-WST can quantify lactate (the lower limit: 0.02 mmol/l), one of the glycolysis metabolites. Because the Lactate Assay Kit-WST corresponds to 96 well micro-plates, it allows for multiple samples to be measured at once. Lactate quantification has been widely used as a marker for monitoring changes in intracellular metabolic pathway in many different areas of cancer research. In recent years, there have been an increasing number of reports on using lactate as an indicator of stem cell differentiation or as an indicator in diabetic research.
The kit is used to detect lactate in cell culture medium by measuring the absorbance of WST formazan produced according to quantities of lactate. The kit includes a “Lactate Standard” which can be used to quantify the concentration of lactate found within samples by creating standard curve.
Simple Assay Procedure
Dojindo’s stock solution is stable for 4 months under refrigerator. Your assay can be done anytime without the need freeze the product and wait for defrosting.
Standard curve can be prepared by using Lactate Standard included in the kit. The concentration of lactate can be measured. The concentration of lactate can be evaluated by diluting the samples if the concentration is over 1 mmol/l.
1) Sample: C2C12
Publication: S. Hori, Y. Hiramuki, D. Nishimura, F. Sato and A. S. Fujisawa, PDH-mediated metabolic flow is critical for skeletal muscle stem cell differentiation and myotube formation during regeneration in mice.", FASEB J. ., 2019, 33(7), 8094.
2) Sample: A2780, OVC
Publication: Y. Nonomiya, K. Noguchi, K. Katayama and Y. Sugimoto, Novel pharmacological effects of poly (ADP-ribose) polymerase inhibitor rucaparib on the lactate dehydrogenase pathway.", Biochem. Biophys. Res. Commun., 2019, 510(4), 510.
3) Sample: HCT116
Publication: K. Ohshima, S. Nojima, S. Tahara, M. Kurashige, K. Kawasaki, Y. Hori, M. Taniguchi, Y. Umakoshi, D. Okuzaki, N. Wada, J. Ikeda, E. Fukusaki and E. Morii, Serine racemase enhances growth of colorectal cancer by producing pyruvate from serine", Nat Metab, 2020, 2(1), 81.
4) Sample: Blood cell, Lymphocytes
Publication: Y. akashima, A. Hayano and B. Yamanaka, Metabolome analysis reveals excessive glycolysis via PI3K/AKT/mTOR and RAS/MAPK signaling in methotrexate-resistant primary CNS lymphoma-derived cells.", Cancer Sci.., 2020, 111(4), 1241-1253.
5) Sample: Lung cancer
Publication: H. Osumi, H. Horiguchi, T. Kadomatsu, K. Tashiro, J. Morinaga, T. Takahashi, K. Ikeda, T. Ito, M. Suzuki, M. Endo and Y. Oike, "Tumor Cell-Derived Angiopoietin-Like Protein 2 Establishes a Preference for Glycolytic Metabolism in Lung Cancer Cells”, Nat Metab, 2020, 2(1), 81
6) Sample: P388
Publication: T. Matsuo, Y. Konya, E. Hirayama and Y. Sadzuka , "2-Deoxy-D-glucose enhances the anti-cancer effects of idarubicin on idarubicin-resistant P388 leukemia cells”, Oncol Lett , 2020, 20(1), 962-966